Journal: Regulatory toxicology and pharmacology : RTP

Article Title: Toxicological comparison of cigarette smoke and e-cigarette aerosol using a 3D in vitro human respiratory model.

doi: 10.1016/j.yrtph.2019.01.036

Figure Lengend Snippet: Figure 4. IL-6 inflammatory cytokine secretion following exposure to cigarette smoke (a), flavored e-cigarette aerosol (b) or base e-liquid aerosol (c). EpiAirway tissues were exposed to three doses of conventional cigarette smoke or base e-liquid aerosol containing 2.4% nicotine with or without blueberry flavor in parallel to control air. Concentration of IL-6 in the media 24 hours after exposure was measured by ELISA and expressed in absolute values with individual matched air-exposed tissues. Mean ± s.d. is shown (n=3, *p-value ≤ 0.05 comparing smoke/aerosol to matched air exposure at each dose).

Article Snippet: 278 Media samples were briefly centrifuged to pellet any debris and the supernatants were stored 279 at -80°C until analysis using the Quantikine ELISA kits according to the manufacturer’s 280 protocols (R&D Systems, Minneapolis, MN, USA).

Techniques: Aerosol, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Regulatory toxicology and pharmacology : RTP

Article Title: Toxicological comparison of cigarette smoke and e-cigarette aerosol using a 3D in vitro human respiratory model.

doi: 10.1016/j.yrtph.2019.01.036

Figure Lengend Snippet: Figure 5. IL-8 inflammatory cytokine secretion following exposure to cigarette smoke (a), flavored e-cigarette aerosol (b) or base e-liquid aerosol (c). EpiAirway tissues were exposed to three doses of conventional cigarette smoke or base e-liquid aerosol containing 2.4% nicotine with or without blueberry flavor in parallel to control air. Concentration of IL-8 in the media 24 hours after exposure was measured by ELISA and expressed in absolute values with individual matched air-exposed tissues. Mean ± s.d. is shown (n=3, *p-value ≤ 0.05 comparing smoke/aerosol to matched air exposure at each dose).

Article Snippet: 278 Media samples were briefly centrifuged to pellet any debris and the supernatants were stored 279 at -80°C until analysis using the Quantikine ELISA kits according to the manufacturer’s 280 protocols (R&D Systems, Minneapolis, MN, USA).

Techniques: Aerosol, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Regulatory toxicology and pharmacology : RTP

Article Title: Toxicological comparison of cigarette smoke and e-cigarette aerosol using a 3D in vitro human respiratory model.

doi: 10.1016/j.yrtph.2019.01.036

Figure Lengend Snippet: Figure 6. Oxidative stress marker, 8-isoprostane, release following exposure to cigarette smoke (a), flavored e-cigarette aerosol (b) or base e-liquid aerosol (c). EpiAirway tissues were exposed to three doses of conventional cigarette smoke or base e- liquid aerosol containing 2.4% nicotine with or without blueberry flavor in parallel to control air. Concentration of 8-isoprostane in the media 24 hours after exposure was measured by ELISA and expressed in absolute values with individual matched air- exposed tissues. Mean ± s.d. is shown (n=3, *p-value ≤ 0.05 comparing smoke/aerosol to matched air exposure at each dose).

Article Snippet: 278 Media samples were briefly centrifuged to pellet any debris and the supernatants were stored 279 at -80°C until analysis using the Quantikine ELISA kits according to the manufacturer’s 280 protocols (R&D Systems, Minneapolis, MN, USA).

Techniques: Marker, Aerosol, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay